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Ito Genta

    Faculty of Pharmaceutical Sciences Associate Professor
    Graduate School of Pharmaceutical Sciences Associate Professor
Last Updated :2025/05/01

Researcher Information

Affiliation

    Faculty of Pharmaceutical Sciences Associate Professor
    Graduate School of Pharmaceutical Sciences Associate Professor

Degree

  • Ph.D. (Pharmaceutical Sciences)（2010/03 The University of Tokyo）
  • MSc (Pharmaceutical Sciences)（2005/03 The University of Tokyo）
  • BSc (Pharmaceutical Sciences)（2003/03 The University of Tokyo）

URL

Researcher ID

  • L-2581-2015

J-Global ID

Profile

  • I started my research in the pathobiochemistry of Parkinson's disease, a major neurodegenerative disease, in the laboratory of Professor Takeshi Iwatsubo (Graduate School of Pharmaceutical Sciences, The University of Tokyo). After working in the laboratories of Professor Dario Alessi (University of Dundee, Scotland) and Professor Taisuke Tomita (Graduate School of Pharmaceutical Sciences, The University of Tokyo). I moved to the laboratory of Professor Naoko Tate (Faculty of Pharmaceutical Sciences, Teikyo University) in 2021. I am still working on elucidating the pathogenic mechanism of Parkinson's and Alzheimer's diseases by analyzing the function and properties of proteins related to these diseases.


    In particular, from the time I was a graduate student to the present, my research has focused on leucine-rich repeat kinase 2 (LRRK2) kinase (Ito et al, Biochemistry, 2006; Kamikawaji et al., Biochemistry, 2009; Ito and Iwatsubo, Biochem J, 2012; Kamikawaji et al., Biochemistry, 2013; Ito et al., PLOS One, 2014). It is a molecule that has been strongly suggested to play an important role in the pathogenesis of Parkinson's disease (PD), as it has been shown to be associated with the risk of developing not only familial PD with genetic mutations, but also sporadic PD in genome-wide association studies.


    Although I have shown that LRRK2 phosphorylates several Rab proteins, vesicular transport-related small GTPases (Steger et al., eLife, 2016; Ito et al., Biochem J, 2016; Fan et al., Biochem J, 2018), the significance of phosphorylation in the regulation of Rab function and its role in the pathogenesis of PD remains unclear. Currently, I am working on the physiological function of LRRK2 (Araki et al., Hum Mol Genet, 2021), the mechanism by which abnormal activation of LRRK2 occurs in PD (Eguchi et al., PNAS, 2018; Ito-Nagai et al, JBC, 2022), and the resulting abnormal Rab protein phosphorylation cause neurodegeneration (Ito K et al., FASEB J, 2023; Ito G et al., BBRC, 2023).


    Since moving to Teikyo University, I have also begun to use biochemical and physicochemical methods to elucidate the effects of amino acid D-isomerization on the pathogenesis of neurodegenerative diseases, which is the main research topic of our laboratory. Amino acid D-isomerization is a phenomenon that occurs non-enzymatically in amino acids including aspartic acid in vivo and increases with age. As such, it may trigger fibril formation of amyloid-β and tau proteins, which accumulate in the brain as amyloids in Alzheimer's disease.


    Amino acid D-isomerization in proteins is ubiquitous in bacterial cell wall synthesis, but its role in higher organisms, the extent to which it occurs, and its physiological and pathological significance are completely unknown. I am working to develop a method to comprehensively analyze amino acid D-isomerization in proteins using proteomics techniques developed in my LRRK2 research.

Research Interests

  • LRRK2   Leucine-rich repease kinase 2   Parkinson's disease   Alzheimer's disease   Amyloid β   kinase   GTP-binding protein   

Research Areas

  • Life sciences / Structural biochemistry
  • Life sciences / Functional biochemistry
  • Life sciences / Neuropathology
  • Life sciences / Neurology
  • Life sciences / Cell biology
  • Life sciences / Pharmaceuticals - health and biochemistry

Academic & Professional Experience

  • 2025/04 - Today  Teikyo UniversityFaculty of Pharmaceutical SciencesAssociate Professor
  • 2021/07 - 2025/03  Teikyo UniversityFaculty of Pharmaceutical SciencesSenior Assistant Professor
  • 2021/07 - 2024/03  The University of TokyoGraduate School of Pharmaceutical Sciences
  • 2021/07 - 2024/03  The University of TokyoFaculty of Pharmaceutical SciencesVisiting Scientist
  • 2017/09 - 2021/06  University of TokyoGraduate School of Pharmaceutical SciencesProject Lecturer
  • 2016/07 - 2017/08  The University of TokyoProject Assistant Professor
  • 2013/08 - 2016/06  University of DundeeMRC Protein Phosphorylation and Ubiquitylation UnitPostdoctral Research Assistant
  • 2008/04 - 2013/07  University of TokyoGraduate School of MedicineResearch Associate
  • 2007/04 - 2008/03  University of TokyoGraduate School of Pharmaceutical SciencesResearch Associate
  • 2006/04 - 2007/03  The University of TokyoGraduate School of Pharmaceutical SciencesResearch Associate

Education

  • 2003/04 - 2006/03  University of Tokyo  Graduate School of Pharmaceutical Sciences
  • 1999/04 - 2003/03  University of Tokyo  School of Pharmaceutical Sciences

Association Memberships

  • Japanese Electrophoresis Society   Japanese Society for Dementia Research   The Pharmaceutical Society of Japan   The Japan Neuroscience Society   The Molecular Biology Society of Japan   Society for Neuroscience   The Japanese Biochemical Society   

Research Activity Information

Published Papers

Books etc

Conference Activities & Talks

  • パーキンソン病原因キナーゼLRRK2によるリソソーム局在制御機構の解明
    伊藤 弦太; 伊藤恭平; 荒木美保; 片井悠太; 西村佑紀; 芋谷颯太; 井上晴幾; 楯直子; 富田泰輔
    第46回日本分子生物学会年会  2023/12
  • Comprehensive identification of proximal proteins and functional analysis of Rab29
    Lejia Xu; Genta Ito; Taisuke Tomita
    Neuroscience 2023
  • Regulation of intracellular lysosomal positioning by LRRK2
    Kyohei Ito; Miho Araki; Yuta Katai; Yuki Nishimura; Sota Imotani; Haruki Inoue; Genta Ito; Taisuke Tomita
    Neuroscience 2023
  • Rab12のヌクレオチド結合状態がLRRK2によるリン酸化と二次構造に与える影響
    伊藤 弦太; 富田 泰輔; 楯 直子
    第96回日本生化学会大会  2023/10
  • パーキンソン病関連タンパク質Rab12の二次構造とLRRK2によるリン酸化に関する解析
    伊藤 弦太; 中村 亮; 楯 直子
    日本薬学会第143年会  2023/03
  • パーキンソン病リスク遺伝子Rab29近傍タンパク質の網羅的同定と機能解析
    徐 楽佳; 伊藤 弦太; 富田 泰輔
    第95回日本生化学会大会  2022/11
  • プロテアーゼを利用したRab29ヌクレオチド結合状態解析系の確立
    梶原 陽太郎; 伊藤 弦太; 富田 泰輔
    第95回日本生化学会大会  2022/11
  • パーキンソン病原因遺伝子LRRK2が引き起こすリソソーム局在変化
    伊藤 恭平; 荒木 美保; 片井 悠太; 西村 佑紀; 井上 晴幾; 伊藤 弦太; 富田 泰輔
    第95回日本生化学会大会  2022/11
  • Analysis of the secondary structure of Rab12, a protein implicated in the pathogenesis of Parkinson’s disease
    伊藤 弦太; 中村 亮; 楯 直子
    第95回日本生化学会大会  2022/11
  • プロテアーゼを利用したRab29ヌクレオチド結合状態解析系の確立
    梶原 陽太郎; 伊藤 弦太; 富田 泰輔
    2022年度日本生化学会関東支部例会  2022/06
  • LRRK2によるリソソームの細胞内局在制御
    伊藤 弦太; 伊藤 恭平; 荒木 美保; 片井 悠太; 西村 佑紀; 井上 晴幾; 富田 泰輔
    日本薬学会第142年会  2022/03
  • BORCS6はLrrk2ノックアウトマウスにおける層板小体肥大化に関与する
    Miho Araki; Kyohei Ito; Sho Takatori; Genta Ito; Taisuke Tomita
    第44回日本分子生物学会年会  2021/12
  • 自閉症モデルマウスにおけるシナプス形成異常関連分子Lingo2の代謝機構解析
    Shiyori Shun; Fumiaki Yoshida; Genta Ito; Sho Takatori; Taisuke Tomita
    第44回日本分子生物学会年会  2021/12
  • LRRK2によるリソソーム局在化制御と生物学的意義
    伊藤 弦太; 伊藤 恭平; 富田 泰輔
    第40回日本認知症学会学術集会  2021/11
  • パーキンソン病リスク遺伝子Rab29の生化学的解析
    長井 ゆき; 伊藤 弦太; 富田 泰輔
    第40回日本認知症学会学術集会  2021/11
  • 電気泳動がひも解くパーキンソン病病因タンパク質の性状と機能
    伊藤 弦太
    第71回日本電気泳動学会シンポジウム  2021/10
  • Regulation of intracellular lysosomal positioning by LRRK2
    Kyohei Ito; Genta Ito; Taisuke Tomita
    第43回日本分子生物学会年会  2020/12
  • Proximity labeling of Rab29 revealed its potential involvement in the ER-Golgi trafficking
    Lejia Xu; Genta Ito; Taisuke Tomita
    第43回日本分子生物学会年会  2020/12
  • アルツハイマー病リスク因子TREM2/DAP12と近接相互作用するタンパク質の解明
    木村 新伍; 高鳥 翔; 井口 明優; 荒木 美保; 伊藤 弦太; 富田 泰輔
    第38回日本認知症学会学術集会
  • Proteomic analysis of lamellar bodies in Lrrk2 knockout mice
    Miho Araki; Sho Takatori; Genta Ito; Taisuke Tomita
    Neuroscience 2019  2019/10
  • Systematic analysis on the seeding activity of familial mutant forms of α-synuclein
    Taisuke Tomita; Ning Xu; Airi Tarutani; Genta Ito
    Neuroscience 2019  2019/10
  • Physiological role of LRRK2 in lamellar body homeostasis
    Genta Ito; Miho Araki; Sho Takatori; Taisuke Tomita
    第92回日本生化学会大会  2019/09
  • バルプロ酸母体投与自閉症モデルマウスにおいて興奮性シナプス形成異常をもたらす分子メカニズムの解明
    Fumiaki Yoshida; Ryota Nagatomo; Misaki Kimura; Genta Ito; Sho Takatori; Taisuke Tomita
    第92回日本生化学会大会  2019/09
  • Characterization of Rab phosphorylation by LRRK kinases
    Genta Ito; Kyohei Ito; Miho Araki; Taisuke Tomita
    The 5th World Parkinson Congress
  • Systematic analysis on the seeding activity of familial mutant forms of α-synuclein
    Ning Xu; Genta Ito; Airi Tarutani; Taisuke Tomita
    The 9th Federation of the Aisan and Oceanian Physiological Societies
  • Characterization of Rab phosphorylation by LRRK kinases
    Genta Ito; Miho Araki; Kyohei Ito; Taisuke Tomita
    第41回日本分子生物学会年会
  • Physiological role of LRRK2 in lamellar body homeostasis
    Miho Araki; Sho Takatori; Genta Ito; Taisuke Tomita
    第41回日本分子生物学会年会
  • バルプロ酸母体投与自閉症モデルマウスにおいて興奮性シナプス形成異常をもたらす分子メカニズムの解明
    Fumiaki Yoshida; Ryota Nagatomo; Misaki Kimura; Genta Ito; Sho Takatori; Taisuke Tomita
    第41回日本分子生物学会年会
  • Phos-tag SDS-PAGEを用いたRabリン酸化の解析
    伊藤 弦太; 富田 泰輔
    第69回日本電気泳動学会総会  2018/08
  • Physiological role of LRRK2 in lamellar body homeostasis
    Miho Araki; Sho Takatori; Genta Ito; Taisuke Tomita
    2018年度日本生化学会関東支部例会  2018/06
  • 糖クリックケミストリーを用いたセクレトーム解析による新規自閉症バイオマーカーの探索
    長友 亮太; 名尾 洋亮; 木村 美咲; 伊藤 弦太; 高鳥 翔; 富田 泰輔
    ConBio2017
  • Phos-tag analysis of Rab10 phosphorylation by LRRK2
    Genta Ito
    第40回日本神経科学大会  2017/07
  • Phos-tag analysis of Rab10 phosphorylation by LRRK2
    Genta Ito
    第39回日本分子生物学会年会
  • Identification of in vivo autophosphorylation sites of LRRK2
    Shogo Kamikawaji; Genta Ito; Takeshi Iwatsubo
    Neuroscience 2012
  • Molecular mechanism of hypophosphorylation of LRRK2 caused by familial mutations
    Genta Ito; Shogo Kamikawaji; Takeshi Iwatsubo
    Neuroscience 2012
  • Identification and functional characterization of novel autophosphorylation sites of LRRK2
    Genta Ito; Shogo Kamikawaji; Takeshi Iwatsubo
    Neuroscience 2011
  • Biochemical characterization of the dimerization of LRRK2
    Genta Ito; Takeshi Iwatsubo
    Neuroscience 2010
  • Rotenone treatment enhances apoptosis caused by G2019S familial Parkinson mutant LRRK2
    Hayato Kunugi; Genta Ito; Takeshi Iwatsubo
    Neuroscience 2010
  • LRRK2による神経細胞死機構の探索
    功刀 隼人; 伊藤 弦太; 岩坪 威
    第28回日本認知症学会学術集会
  • 家族性パーキンソン病責任遺伝子産物LRRK2の新規基質探索
    新留 一樹; 伊藤 弦太; 岩坪 威
    第29回日本認知症学会学術集会
  • Autophosphorylation in the kinase domain of LRRK2: relevance to kinase activity
    Genta Ito; Shogo Kamikawaji; Takeshi Iwatsubo
    Neuroscience 2009
  • Systematic characterization of autophosphorylation of LRRK2
    Shogo Kamikawaji; Genta Ito; Takeshi Iwatsubo
    Neuroscience 2009
  • Functional characterization of phosphorylation of LRRK2
    Genta Ito; Shogo Kamikawaji; Kazuki Niidome; Takeshi Iwatsubo
    Neuroscience 2008
  • LRRK2自己リン酸化部位の同定
    上川路 翔吾; 伊藤 弦太; 岩坪 威
    第27回日本認知症学会学術集会
  • Characterization of phosphorylation of LRRK2
    Genta Ito; Shogo Kamikawaji; Takeshi Iwatsubo
    Neuroscience 2007
  • Relationship between GTP binding and protein kinase activity of LRRK2
    Genta Ito; Takuro Okai; Go Fujino; Kosuke Takeda; Hidenori Ichijo; Toshiaki Katada; Takeshi Iwatsubo
    Neuroscience 2006
  • Molecular and cell biological studies on the antioxidant function of DJ-1, a causative gene for parkinsonism
    Genta Ito; Hiroyoshi Ariga; Yasukazu Nakagawa; Makoto Miyagishi; Kazunari Taira; Tomohiko Maehama; Takeshi Iwatsubo
    Neuroscience 2005
  • Molecular and cell biological studies on the metabolism and function of DJ-1, a novel causative gene for parkinsonism
    Genta Ito; Hiroyoshi Ariga; Yasukazu Nakagawa; Makoto Miyagishi; Kazunari Taira; Takeshi Iwatsubo
    第77回日本生化学会大会

MISC

Research Grants & Projects

Teaching Experience

  • 薬の現在・過去・未来（共通教育科目）薬の現在・過去・未来（共通教育科目） Teikyo University