Shimozono Satoshi

Researcher Information

Affiliation

Faculty of Pharmaceutical Sciences Professor

URL

https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201301051107782030

Research funding number

• 40391982

ORCID ID

•  https://orcid.org/0000-0001-6803-5961

J-Global ID

• 201301051107782030

Research Interests

• 生体内分析学   バイオイメージング   蛍光タンパク質   retinoic acid   Nuclear receptors   細胞分裂   神経   核膜   拡散   

Research Areas

• Life sciences / Pharmaceuticals - analytical and physicochemistry
• Life sciences / Pharmaceuticals - health and biochemistry
• Life sciences / Cell biology

Academic & Professional Experience

• 2025/07 - Today  RIKEN
• 2025/04 - Today  Teikyo UniversityFaculty of Pharmaceutical Sciences
• 1994 - 1998  The University of TokyoFaculty of Pharmaceutical Sciences

Association Memberships

• The Biophysical Society of Japan   The Pharmaceutical Society of Japan   日本レチノイド研究会   

Research Activity Information

Published Papers

• Femtosecond‐Laser‐Fabricated 3D CYTOP Microfluidic Chips Enabling Super‐Resolution Live Imaging of Cell Behavior in Confined Microspaces

  Koji Sugioka; Mirai Hanzawa; Kotaro Obata; Sota Matsumoto; Masatoshi Takagi; Asako Sakaue‐Sawano; Asako Tosaki; Satoshi Shimozono; Felix Sima; Hiroyuki Kawano; Takuro Tojima; Daisuke Miyashiro; Kazunari Ozasa; Yasutaka Hanada; Godai Miyaji; Akihiko Nakano; Atsushi Miyawaki

  Advanced Materials Technologies 2026/03
• Spectroscopic Analysis of Salicylate Dianion Generated during Aspirin Titration Based on the Japanese Pharmacopoeia Method.

  Satoshi Shimozono; Hirofumi Konno; Yasushi Kishimoto

  Chemical & pharmaceutical bulletin 74 (6) 466 - 470 2026
• StayGold photostability under different illumination modes.

  Masahiko Hirano; Yasuo Yonemaru; Satoshi Shimozono; Mayu Sugiyama; Ryoko Ando; Yasushi Okada; Takahiro Fujiwara; Atsushi Miyawaki

  Scientific reports 14 (1) 5541 - 5541 2024/03 [Refereed]
  
• Comparison of monomeric variants of StayGold

  Satoshi Shimozono; Ryoko Ando; Mayu Sugiyama; Masahiko Hirano; Yusuke Niino; Atsushi Miyawaki

  2024/02
• StayGold variants for molecular fusion and membrane-targeting applications

  Ryoko Ando; Satoshi Shimozono; Hideo Ago; Masatoshi Takagi; Mayu Sugiyama; Hiroshi Kurokawa; Masahiko Hirano; Yusuke Niino; Go Ueno; Fumiyoshi Ishidate; Takahiro Fujiwara; Yasushi Okada; Masaki Yamamoto; Atsushi Miyawaki

  Nature Methods Springer Science and Business Media LLC 21 (4) 648 - 656 1548-7091 2023/11 [Refereed]
  
• A highly photostable and bright green fluorescent protein

  Masahiko Hirano; Ryoko Ando; Satoshi Shimozono; Mayu Sugiyama; Noriyo Takeda; Hiroshi Kurokawa; Ryusaku Deguchi; Kazuki Endo; Kei Haga; Reiko Takai-Todaka; Shunsuke Inaura; Yuta Matsumura; Hiroshi Hama; Yasushi Okada; Takahiro Fujiwara; Takuya Morimoto; Kazuhiko Katayama; Atsushi Miyawaki

  Nature Biotechnology Springer Science and Business Media LLC 40 (7) 1132 - 1142 1087-0156 2022/07 [Refereed]
  
• Single-cell bioluminescence imaging of deep tissue in freely moving animals.

  Satoshi Iwano; Mayu Sugiyama; Hiroshi Hama; Akiya Watakabe; Naomi Hasegawa; Takahiro Kuchimaru; Kazumasa Z Tanaka; Megumu Takahashi; Yoko Ishida; Junichi Hata; Satoshi Shimozono; Kana Namiki; Takashi Fukano; Masahiro Kiyama; Hideyuki Okano; Shinae Kizaka-Kondoh; Thomas J McHugh; Tetsuo Yamamori; Hiroyuki Hioki; Shojiro Maki; Atsushi Miyawaki

  Science (New York, N.Y.) 359 (6378) 935 - 939 0036-8075 2018/02 [Refereed]
  
• Visualization of an endogenous retinoic acid gradient across embryonic development

  Satoshi Shimozono; Tadahiro Iimura; Tetsuya Kitaguchi; Shin-ichi Higashijima; Atsushi Miyawaki

  NATURE 496 (7445) 363 - + 0028-0836 2013/04 [Refereed]
  
• Diffusion of Large Molecules into Assembling Nuclei Revealed Using an Optical Highlighting Technique

  Satoshi Shimozono; Hidekazu Tsutsui; Atsushi Miyawaki

  BIOPHYSICAL JOURNAL 97 (5) 1288 - 1294 0006-3495 2009/09 [Refereed]
  
• Development of microscopic systems for high-speed dual-excitation ratiometric Ca2+imaging

  Takashi Fukano; Satoshi Shimozono; Atsushi Miyawaki

  BRAIN CELL BIOLOGY 36 (1-4) 43 - 52 1559-7105 2008/08 [Refereed]
  
• Engineering FRET constructs using CFP and YFP

  Satoshi Shimozono; Atsushi Miyawaki

  FLUORESCENT PROTEINS, SECOND EDITION 85 381 - + 0091-679X 2008 [Refereed]
  
• Identification of mitochondrial DNA polymorphisms that alter mitochondrial matrix pH and intracellular calcium dynamics

  An-a Kazuno; Kae Munakata; Takeharu Nagai; Satoshi Shimozono; Masashi Tanaka; Makoto Yoneda; Nobumasa Kato; Atsushi Miyawaki; Tadafumi Kato

  PLOS GENETICS 2 (8) 1167 - 1177 1553-7390 2006/08 [Refereed]
  
• Concatenation of cyan and yellow fluorescent proteins for efficient resonance energy transfer

  S Shimozono; H Hosoi; H Mizuno; T Fukano; T Tahara; A Miyawaki

  BIOCHEMISTRY 45 (20) 6267 - 6271 0006-2960 2006/05 [Refereed]
  
• Fast dual-excitation ratiometry with light-emitting diodes and high-speed liquid crystal shutters

  T Fukano; S Shimozono; A Miyawaki

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 340 (1) 250 - 255 0006-291X 2006/02 [Refereed]
  
• Slow Ca2+ dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping

  S Shimozono; T Fukano; KD Kimura; Mori, I; Y Kirino; A Miyawaki

  EMBO REPORTS 5 (5) 521 - 526 1469-221X 2004/05 [Refereed]
  
• Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights

  T Fukano; S Shimozono; A Miyawaki

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 317 (1) 77 - 83 0006-291X 2004/04 [Refereed]
  
• Optical recording of oscillatory neural activities in the molluscan brain

  S Watanabe; S Shimozono; Y Kirino

  NEUROSCIENCE LETTERS 359 (3) 147 - 150 0304-3940 2004/04 [Refereed]
  
• Confocal imaging of subcellular Ca2+ concentrations using a dual-excitation ratiometric indicator based on green fluorescent protein.

  Shimozono S; Fukano T; Nagai T; Kirino Y; Mizuno H; Miyawaki A

  Science's STKE : signal transduction knowledge environment 125 2002 pl4  2002/03 [Refereed]
  
• Identification and characterization of an output neuron from the oscillatory molluscan olfactory network

  S Shimozono; S Watanabe; T Inoue; Y Kirino

  BRAIN RESEARCH 921 (1-2) 98 - 105 0006-8993 2001/12 [Refereed]
  

Conference Activities & Talks

• 生体内レチノイン酸分布の可視化

  下薗哲

  第126回日本解剖学会 総会・ 全国学術集会 第98回日本生理学会大会  2021/03
• Visualization of retinoic acid gradients

  Satoshi SHIMOZONO; Atsushi MIYAWAKI

  第57回日本生物物理学会  2019/09
• 生体内レチノイン酸グラディエントの定量的可視化

  下薗 哲; 宮脇 敦史

  第 347 回 脂溶性ビタミン総合研究委員会  2015/07
• 脂質研究における蛍光タンパク質技術

  下薗 哲; 宮脇 敦史

  第53回日本脂質生化学会  2011/05

MISC

• 多角的な蛍光/発光プローブ開発による脳科学の推進

  下薗 哲; 宮脇 敦史  生体の科学  73-  (5)  428  -429  2022/10
• FRETによるタンパク質間相互作用解析

  下薗 哲; 宮脇 敦史  タンパク質実験ノート 下巻  2011/11

Awards & Honors

• 2025/03 国立研究開発法人理化学研究所 理研梅峰賞
   

  受賞者: 安藤 亮子;下薗 哲;高木 昌俊;杉山 真由;黒川 裕;平野 雅彦;新野 祐介
• 2023/03 The Institute of Physical and Chemical Research RIKEN EIHO Award
   Sustainable bioimaging using a fluorescent protein with unprecedented photostability 

  受賞者: 平野 雅彦;下薗 哲;杉山 真由;黒川 裕;安藤 亮子;宮脇 敦史
• 2015/04 長瀬科学技術振興財団 長瀬科学振興賞
   

  受賞者: 下薗 哲

Research Grants & Projects

• Strategic orchestration of bioimaging technologies for understanding heterogeneity in biology

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research

  Date (from‐to) : 2026/04 -2031/03 

  Author : 宮脇 敦史; 阪上ー沢野 朝子; 高木 昌俊; 新野 祐介; 岩野 智; 下薗 哲
• 光安定な蛍光タンパク質の多色化

  日本学術振興会：科学研究費助成事業

  Date (from‐to) : 2026/04 -2029/03 

  Author : 下薗 哲
• Visualization of retinoic acid gradients underlying the mouse limb formation

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

  Date (from‐to) : 2019/04 -2022/03 

  Author : SHIMOZONO Satoshi
• The development of fundamental bioimaging technologies for comprehensive understanding

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Date (from‐to) : 2015/06 -2020/03 

  Author : Atsushi Miyawaki
• Improvement of the genetically encoded probe for retinoic acid, GEPRA.

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

  Date (from‐to) : 2014/04 -2017/03 

  Author : Shimozono Satoshi
• Visualizing biological functions at many different scales

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

  Date (from‐to) : 2010/04 -2015/03 

  Author : MIYAWAKI Atsushi; HAMA Hiroshi; SASAKI Kazuki; SHIMOZONO Satoshi; NIINO Yusuke; SAKAUE Asako; KAWANO Hiroyuki; FUKANO Takashi; ANDO Ryoko
• Possible functions of the permeability barrier of the nuclear envelope.

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

  Date (from‐to) : 2012/04 -2014/03 

  Author : SHIMOZONO Satoshi
• Permeablity change of the nuclear envelope after mitosis.

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)

  Date (from‐to) : 2008 -2009 

  Author : SHIMOZONO Satoshi
• New approaches for imaging and controlling the molecules of life.

  Japan Society for the Promotion of Science：Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Priority Areas

  Date (from‐to) : 2005 -2009 

  Author : MIYAWAKI Atsushi; MIZUNO Hideaki; SHIMOZONO Satoshi; FUKANO Takashi; TSUTSUI Hidekazu; NAGAI Takeharu; IBATA Keiji; MIYAUCHI Takayuki; VETRIVEL Lakshmanan
• 生体内レチノイン酸の可視化

  内藤記念科学振興財団：内藤記念特定研究助成金

  Author : 下薗 哲

Teaching Experience

Analytical chemistryAnalytical chemistry Kitasato University