Fujii Norihiko

Researcher Information

Affiliation

Itabashi Campus Joint Research Facility Senior Assistant Professor

URL

https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201401004758808622

J-Global ID

• 201401004758808622

Research Interests

• peptide   Isomer   Protein   

Research Areas

• Nanotechnology/Materials / Molecular biochemistry

Academic & Professional Experience

• 2013/01 - Today  Teikyo UniversityRadioisotope Research CenterLecturer
• 2008/04 - 2012/12  Kyoto University Research Reactor InstituteResearch Scientist

Education

• 1998/04 - 2002/03  Kansai University  Faculty of Engineering  Department of Applied Chemistry

Association Memberships

• THE PHARMACEUTICAL SOCIETY OF JAPAN   D-AMINO ACID RESEARCH SOCIETY   THE JAPANESE SOCIETY FOR CATARACT RESEARCH   THE JAPANESE BIOCHEMICAL SOCIETY   

Research Activity Information

Published Papers

• One-shot LC-MS/MS analysis of post-translational modifications including oxidation and deamidation of rat lens alpha- and beta-crystallins induced by gamma-irradiation

  Ingu Kim; Takeshi Saito; Norihiko Fujii; Takashi Kanamoto; Noriko Fujii

  AMINO ACIDS 48 (12) 2855 - 2866 0939-4451 2016/12 [Refereed]
  
• New insight into the dynamical system of alpha B-crystallin oligomers

  Rintaro Inoue; Takumi Takata; Norihiko Fujii; Kentaro Ishii; Susumu Uchiyama; Nobuhiro Sato; Yojiro Oba; Kathleen Wood; Koichi Kato; Noriko Fujii; Masaaki Sugiyama

  SCIENTIFIC REPORTS 6 29208  2045-2322 2016/07 [Refereed]
  
• Isomerization of aspartyl residues in crystallins and its influence upon cataract.

  Fujii N; Takata T; Fujii N; Aki K

  Biochimica et biophysica acta 1860 (1 Pt B) 183 - 191 0006-3002 2016/01 [Refereed]
  
• Quantitative analysis of isomeric (L-alpha-, L-beta-, D-alpha, D-beta-) aspartyl residues in proteins from elderly donors

  Noriko Fujii; Takumi Takata; Norihiko Fujii

  JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 116 25 - 33 0731-7085 2015/12 [Refereed]
  
• Site specific oxidation of amino acid residues in rat lens gamma-crystallin induced by low-dose gamma-irradiation

  Ingu Kim; Takeshi Saito; Norihiko Fujii; Takashi Kanamoto; Toshiyuki Chatake; Noriko Fujii

  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 466 (4) 622 - 628 0006-291X 2015/10 [Refereed]
  
• Real-time heterogeneous protein-protein interaction between alpha A-crystallin N-terminal mutants and alpha B-crystallin using quartz crystal microbalance (QCM)

  Srinivasagan Ramkumar; Noriko Fujii; Hiroaki Sakaue; Norihiko Fujii; Bency Thankappan; Rasiah Pratheepa Kumari; Kalimuthusamy Natarajaseenivasan; Kumarasamy Anbarasu

  AMINO ACIDS 47 (5) 1035 - 1043 0939-4451 2015/05 [Refereed]
  
• Alpha B- and βA3-crystallins containing d-aspartic acids exist in a monomeric state.

  Sakaue H; Takata T; Fujii N; Sasaki H; Fujii N

  Biochimica et biophysica acta 1 1854 (1) 1 - 9 0006-3002 2015/01 [Refereed]
  
• Rapid Survey of Four Asp Isomers in Disease-Related Proteins by LC-MS combined with Commercial Enzymes

  Hiroki Maeda; Takumi Takata; Norihiko Fujii; Hiroaki Sakaue; Satoru Nirasawa; Saori Takahashi; Hiroshi Sasaki; Noriko Fujii

  ANALYTICAL CHEMISTRY 87 (1) 561 - 568 0003-2700 2015/01 [Refereed]
  
• Comparison of effect of gamma ray irradiation on wild-type and N-terminal mutants of αA-crystallin.

  Ramkumar S; Fujii N; Fujii N; Thankappan B; Sakaue H; Ingu K; Natarajaseenivasan K; Anbarasu K

  Molecular vision 20 1002 - 1016 2014/07 [Refereed]
  
• Simultaneous ultraviolet B-induced photo-oxidation of tryptophan/tyrosine and racemization of neighboring aspartyl residues in peptides

  Simin Cai; Norihiko Fujii; Takeshi Saito; Noriko Fujii

  FREE RADICAL BIOLOGY AND MEDICINE 65 1037 - 1046 0891-5849 2013/12 [Refereed]
  
• Kinetics of Isomerization and Inversion of Aspartate 58 of alpha A-Crystallin Peptide Mimics under Physiological Conditions

  Kenzo Aki; Norihiko Fujii; Noriko Fujii

  PLOS ONE 8 (3) e58515  1932-6203 2013/03 [Refereed]
  
• A Rapid, Comprehensive Liquid Chromatography-Mass Spectrometry (LC-MS)-based Survey of the Asp Isomers in Crystallins from Human Cataract Lenses

  Norihiko Fujii; Hiroaki Sakaue; Hiroshi Sasaki; Noriko Fujii

  JOURNAL OF BIOLOGICAL CHEMISTRY 287 (47) 39992 - 40002 0021-9258 2012/11 [Refereed]
  
• Characterization of an antibody that recognizes peptides containing D-beta-aspartyl residues

  Kenzo Aki; Norihiko Fujii; Takeshi Saito; Noriko Fujii

  MOLECULAR VISION 18 (104-07) 996 - 1003 1090-0535 2012/04 [Refereed]
  
• UV B-irradiation enhances the racemization and isomerizaiton of aspartyl residues and production of N-epsilon-carboxymethyl lysine (CML) in keratin of skin

  Yuhei Mori; Kenzo Aki; Katsunori Kuge; Shingo Tajima; Natsuko Yamanaka; Yuichi Kaji; Naoki Yamamoto; Ryoji Nagai; Hanako Yoshii; Norihiko Fujii; Masami Watanabe; Tadatoshi Kinouchi; Noriko Fujii

  JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879 (29) 3303 - 3309 1570-0232 2011/11 [Refereed]
  
• Simultaneous Stereoinversion and Isomerization at the Asp-4 Residue in beta B2-Crystallin from the Aged Human Eye Lenses

  Norihiko Fujii; Takehiro Kawaguchi; Hiroshi Sasaki; Noriko Fujii

  BIOCHEMISTRY 50 (40) 8628 - 8635 0006-2960 2011/11 [Refereed]
  
• Substrate stereoselectivity of mammalian D-aspartyl endopeptidase

  Tadatoshi Kinouchi; Norihiko Fujii; Noriko Fujii

  JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879 (29) 3349 - 3352 1570-0232 2011/11 [Refereed]
  
• Influence of L beta-, D alpha- and D beta-Asp isomers of the Asp-76 residue on the properties of alpha A-crystallin 70-88 peptide

  Noriko Fujii; Norihiko Fujii; Masashi Kida; Tadatoshi Kinouchi

  AMINO ACIDS 39 (5) 1393 - 1399 0939-4451 2010/11 [Refereed]
  
• Differentiation and Semiquantitative Analysis of an Isoaspartic Acid in Human alpha-Crystallin by Postsource Decay in a Curved Field Reflectron

  Yuzo Yamazaki; Norihiko Fujii; Yutaka Sadakane; Noriko Fujii

  ANALYTICAL CHEMISTRY 82 (15) 6384 - 6394 0003-2700 2010/08 [Refereed]
  
• Collapse of Homochirality of Amino Acids in Proteins from Various Tissues during Aging

  Noriko Fujii; Yuichi Kaji; Norihiko Fujii; Tooru Nakamura; Ryota Motoie; Yuhei Mori; Tadatoshi Kinouchi

  CHEMISTRY & BIODIVERSITY 7 (6) 1389 - 1397 1612-1872 2010 [Refereed]
  
• SAXS and SANS Observations of Abnormal Aggregation of Human alpha-Crystallin

  Masaaki Sugiyama; Norihiko Fujii; Yukio Morimoto; Keiji Itoh; Kazuhiro Mori; Toshiharu Fukunaga; Noriko Fujii

  CHEMISTRY & BIODIVERSITY 7 (6) 1380 - 1388 1612-1872 2010 [Refereed]
  
• Oxidative Stress Induces the Formation of D-Aspartyl Residues in the Elastin Mimic Peptides

  Katsunori Kuge; Keiko Kitamura; Koichi Nakaoji; Kazuhiko Hamada; Norihiko Fujii; Takeshi Saito; Noriko Fujii

  CHEMISTRY & BIODIVERSITY 7 (6) 1408 - 1412 1612-1872 2010 [Refereed]
  
• Localization of D-beta-Aspartyl Residue-Containing Proteins in Various Tissues

  Ryota Motoie; Noriko Fujii; Shigeru Tsunoda; Kenji Nagata; Tadashi Shimo-oka; Tadatoshi Kinouchi; Norihiko Fujii; Takeshi Saito; Koji Ono

  INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 10 (5) 1999 - 2009 1422-0067 2009/05 [Refereed]
  
• Study of subunit interactions of alpha A- and alpha B-crystallins and the effects of gamma-irradiation on their interactions by surface plasmon resonance.

  Fujii N; Hisano T; Fujii N

  Biochimica et biophysica acta 11 1784 (11) 1507 - 1513 0006-3002 2008/11 [Refereed]
  
• Differential susceptibility of alpha A- and alpha B-crystallin to gamma-ray irradiation.

  Fujii N; Nakamura T; Sadakane Y; Saito T; Fujii N

  Biochimica et biophysica acta 3 1774 (3) 345 - 350 0006-3002 2007/03 [Refereed]
  
• Characterization of alpha A-crystallin from high molecular weight aggregates in the normal human lens

  N Fujii; M Awakura; L Takemoto; M Inomata; T Takata; N Fujii; T Saito

  MOLECULAR VISION 9 (44) 315 - 322 1090-0535 2003/07 [Refereed]
  

Conference Activities & Talks

• 種々のクリスタリン中のアスパラギン酸残基の異性化と立体構造の関係

  藤井 智彦; 佐々木; 洋 藤井 紀子

  第４０回水晶体研究会  2014/01
• LC-MSを用いたタンパク質中のＤアスパラギン酸残基の新しい分析方法

  藤井 智彦; 藤井; 紀子

  第９回D-アミノ酸研究会学術講演会  2013/09
• LC-MSによる白内障の水晶体蛋白質中のアスパラギン酸残基の異性体の分析

  藤井 智彦; 藤井; 紀子

  第５２回日本白内障学会総会  2013/06
• LC-MSによる新規アスパラギン酸残基の異性体の分析

  藤井 智彦; 佐々木; 洋 藤井 紀子

  第３９回水晶体研究会  2013/01

MISC

• Analysis of d-β-aspartyl isomers at specific sites in proteins

  Noriko Fujii; Norihiko Fujii  Methods in Molecular Biology  794-  325  -335  2012  [Refereed]
  
• D-Amino acids in aged proteins: Analysis and biological relevance

  Noriko Fujii; Yuichi Kaji; Norihiko Fujii  JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES  879-  (29)  3141  -3147  2011/11  [Refereed]
  

Awards & Honors

• 2012/06 THE JAPANESE SOCIETY FOR CATARACT RESEARCH prize from an academic society
   水晶体蛋白質中のD-アミノ酸を指標とした 白内障発症機構の解明に関する研究 

  受賞者: 藤井 智彦